FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_15.35100000042104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_15.35100000042104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218571
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC8774240.14347740551125No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT5507325.19684679120286No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG53202.4339917006373213No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG4140.18941213610222765No Hit
TTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTCT2450.11209172305566611RNA PCR Primer, Index 13 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTT207.0249615E-444.9945078
TGCCGTC91800.044.43085545
CCCGTAT356.2303116E-638.56672340
GCCGTCT1100.036.8136945
GATTTTG250.00210464535.99560515
GAAAGGG250.00210464535.9956059
TCTTATA77800.034.1029741
AATCCCG352.8149714E-432.13893537
AAATCCC352.8149714E-432.13893536
TCCCGTA352.8149714E-432.13893539
TGCAGTC300.00514131529.99633845
CCGTATG406.154439E-428.12156741
ATGCCGT149600.027.27942544
TATGCCG154650.026.40318143
CGTATGC155200.026.38209241
GTATGCC154950.026.381142
CTCGTAT154900.026.3750939
TCGTATG155150.026.36159340
TCTCGTA154950.026.3520638
ATCTCGT154800.026.3049337
Produced by FastQC (version 0.11.2)