FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_14.351000000420f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_14.351000000420f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363397
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC75172.0685366142263146No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT25150.6920805620299562No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4770.13126140281840523No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.12383151209283512No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4480.12328114981686696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8900.043.98768645
ATCGCCC301.13855014E-437.4990826
ACCGTGC250.002105469135.9991238
TCGACTA250.002105469135.99912345
ATGCCGT11200.034.95450244
CTCGTAT11600.034.13709639
CACGAGA11800.033.74917221
CCACGAG11800.033.74917220
TCTCGTA11750.033.5098238
CGAGACA11850.033.41690423
ATCTCGT11800.033.36782537
TATGCCG11800.033.36782543
GACATAG11800.033.36782526
CGTATGC11900.033.27649741
TCGTATG11900.033.27649740
ACGAGAC11900.033.27649722
AATCTCG11900.033.08742536
CATAGAC12050.032.6755528
CCCACGA12300.032.56017719
AAATCTC12100.032.54052435
Produced by FastQC (version 0.11.2)