FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_13.351000000420cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_13.351000000420cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464832
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC85821.846258433154344No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT11090.23858082059754923No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7530.1619940107393639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.1445683601817431No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6160.1325209968332645No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.12219468539171141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.10799600715957593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9100.042.52371245
ATGCCGT10750.035.99681544
TATGCCG11050.035.01952743
CGAGACA11200.034.95226723
ACGAGAC11200.034.95226722
CCACGAG11200.034.95226720
CTCGTAT11200.034.55051439
CCCACGA11500.034.23610319
GACATAG11550.034.0878926
GTATGCC11450.033.99262642
CGTATGC11500.033.84483341
CATAGAC11650.033.79529628
CACGAGA11600.033.74701321
TCTCGTA11500.033.64919738
TCGTATG11500.033.64919740
GCCCACG11800.033.3656918
ATAGACA11850.033.22490729
ATCTCGT11700.032.88170637
AGACATA11950.032.75860625
AGCCCAC12050.032.6734617