FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_12.351000000420b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_12.351000000420b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411621
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC158243.8443130938411794No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT20440.496573304083125No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19090.4637761435883981No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14250.3461922496665622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.3214121728483241No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.3063497732137087No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10580.257032561506823No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.23565367170285287No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8520.2069865240111656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.18925176315105402No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7270.1766187828123444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.17103111843176125No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6550.15912696388182332No Hit
ATATGAAACTGTTGAAAAATGAAAATCAAAATTGAAATTCAGTAAGACTTT6320.1535392995012402No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5830.14163514495130228No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5780.14042043530334944No Hit
ATATAAAGTCAAAGCAATGTGAAGAATACTAGTGGCACAATTGTTATTGGA5540.13458982899317576No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG5200.1263298033870964No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.11977037128815099No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4320.10495091358312622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC16850.044.19654545
ATGCCGT19100.038.99014744
TATGCCG19600.037.99550243
CGTATGC19900.037.53576341
CGAGACA19900.037.53576323
ACGAGAC20000.037.46058322
ATCTCGT19900.037.42270337
CACGAGA20050.037.36715721
CCACGAG20050.037.36715720
TCGTATG20000.037.2355940
CCCACGA20150.037.18171719
CTCGTAT20050.037.1427339
AATCTCG20050.037.1427336
TCTCGTA20200.036.8669238
ATAGACA20050.036.8060929
TAGACAA20100.036.7145330
GTATGCC20350.036.70573442
AGACATA20350.036.70573425
GAGACAT20450.036.52624524
CATAGAC20350.036.48461528
Produced by FastQC (version 0.11.2)