FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_1.35100000041f5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_1.35100000041f5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340063
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC32970.96952623484472No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4980.14644345312486215No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT4070.119683705666303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC302.1550513E-645.0179441
TTTATAG253.8792226E-545.0113263
TCTAGCA356.2245526E-638.5811352
AGACAAT4200.034.2842625
CTAGCAC401.5546679E-533.7584953
GTATGCC4250.033.35152442
TATCTCG4400.033.23722536
AATGCGT4350.033.1020429
CTCGTAT4450.032.8637739
GCGTTAT4550.032.63597532
TGCGTTA4500.032.49861531
CACGAGA4650.031.93412621
GACAATG4600.031.79212826
CGTATGC4550.031.64700741
TGCCGTC4500.031.4986645
CGTTATC4650.031.45027533
CCACGAG4750.031.2618320
GAGACAA4650.030.96642524
CAATGCG4800.030.93618428
TATGCCG4600.030.81390843
Produced by FastQC (version 0.11.2)