Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pa_96.35100000041f30.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3537 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC | 242 | 6.841956460277071 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT | 16 | 0.45236075770426915 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG | 9 | 0.2544529262086514 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTC | 7 | 0.19790783149561775 | RNA PCR Primer, Index 26 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT | 6 | 0.16963528413910092 | RNA PCR Primer, Index 18 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATGCCGT | 35 | 8.137795E-8 | 45.0 | 44 |
CGAGCCC | 35 | 8.137795E-8 | 45.0 | 15 |
CCGAGCC | 35 | 8.137795E-8 | 45.0 | 14 |
TCCGAGC | 35 | 8.137795E-8 | 45.0 | 13 |
AGCCCAC | 40 | 2.3272514E-7 | 39.375004 | 17 |
GTATGCC | 40 | 2.3272514E-7 | 39.375004 | 42 |
AATCTCG | 40 | 2.3272514E-7 | 39.375004 | 36 |
ATCTCGT | 40 | 2.3272514E-7 | 39.375004 | 37 |
GCCCACG | 40 | 2.3272514E-7 | 39.375004 | 18 |
TGCCGTC | 40 | 2.3272514E-7 | 39.375004 | 45 |
GAGCCCA | 40 | 2.3272514E-7 | 39.375004 | 16 |
CCCACGA | 40 | 2.3272514E-7 | 39.375004 | 19 |
TCTCGTA | 40 | 2.3272514E-7 | 39.375004 | 38 |
ACGAGAC | 40 | 2.3272514E-7 | 39.375004 | 22 |
CGTATGC | 40 | 2.3272514E-7 | 39.375004 | 41 |
TCGTATG | 40 | 2.3272514E-7 | 39.375004 | 40 |
CGAGACC | 40 | 2.3272514E-7 | 39.375004 | 23 |
AGACCAG | 40 | 2.3272514E-7 | 39.375004 | 25 |
GACCAGC | 40 | 2.3272514E-7 | 39.375004 | 26 |
CTCGTAT | 40 | 2.3272514E-7 | 39.375004 | 39 |