FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_96.35100000041f30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_96.35100000041f30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC2426.841956460277071No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT160.45236075770426915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG90.2544529262086514No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTC70.19790783149561775RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT60.16963528413910092RNA PCR Primer, Index 18 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT358.137795E-845.044
CGAGCCC358.137795E-845.015
CCGAGCC358.137795E-845.014
TCCGAGC358.137795E-845.013
AGCCCAC402.3272514E-739.37500417
GTATGCC402.3272514E-739.37500442
AATCTCG402.3272514E-739.37500436
ATCTCGT402.3272514E-739.37500437
GCCCACG402.3272514E-739.37500418
TGCCGTC402.3272514E-739.37500445
GAGCCCA402.3272514E-739.37500416
CCCACGA402.3272514E-739.37500419
TCTCGTA402.3272514E-739.37500438
ACGAGAC402.3272514E-739.37500422
CGTATGC402.3272514E-739.37500441
TCGTATG402.3272514E-739.37500440
CGAGACC402.3272514E-739.37500423
AGACCAG402.3272514E-739.37500425
GACCAGC402.3272514E-739.37500426
CTCGTAT402.3272514E-739.37500439