FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_92.35100000041eb7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_92.35100000041eb7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11453
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC322028.114904391862396No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT3312.8900724700951717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG890.7770889723216624No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT170.1484327250502052RNA PCR Primer, Index 18 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC140.1222387147472278TruSeq Adapter, Index 4 (95% over 22bp)
CTTATAACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT140.1222387147472278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3850.043.83116545
ATGCCGT4150.040.6626544
AGCCCAC4400.038.8636417
ATCTCGT4400.038.8636437
GAGCCCA4400.038.8636416
TCTCGTA4400.038.8636438
CCGAGCC4400.038.8636414
CTCGTAT4400.038.8636439
GCCCACG4350.038.79310618
TATGCCG4350.038.79310643
CACGAGA4350.038.79310621
CCCACGA4350.038.79310619
ACGAGAC4350.038.79310622
CGAGACC4350.038.79310623
AGACCAG4350.038.79310625
GACCAGC4350.038.79310626
AATCTCG4300.038.72093236
CCAGCAG4300.038.72093228
CAATCTC4300.038.72093235
GCAATCT4300.038.72093234