FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_9.35100000041455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_9.35100000041455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287788
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC48261.6769288504037694No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT8160.2835420517881218No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5770.20049480867861064No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGC250.0021044936.02
TGCCGTC6350.035.0787445
TATGCCG6350.034.7244143
ATGCCGT6350.034.7244144
GTATGCC6400.034.45312542
CACGAGA6500.034.2692321
TCGTATG6450.034.18604740
GACATAG6400.034.10156226
CGTATGC6500.033.92307741
CGAGACA6600.033.7523
AGACATA6700.033.24626525
CTCGTAT6650.033.15789439
TCTCGTA6650.033.15789438
CCCACGA6800.032.75735519
ATAGACA6750.032.66666429
CATAGAC6750.032.66666428
AATCTCG6750.032.66666436
GAGACAT6850.032.5182524
TAGACAA6650.032.481230
GACAAAT6650.032.481232
Produced by FastQC (version 0.11.2)