FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_89.35100000041e4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_89.35100000041e4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100760
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC32533.2284636760619296No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30733.0498213576816195No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA20542.0385073441842No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19931.977967447399762No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA18081.7943628423977769No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13681.3576816196903534No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8700.8634378721714966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.7701468836840016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.7016673283048829No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6600.6550218340611353No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6560.6510520047637951No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5560.5518062723302898No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5390.5349344978165939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.48928146089718144No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.4734021437078206No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4490.44561333862643904No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT4440.44065105200476373No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4350.4317189360857483No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.40591504565303693No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA3700.36720921000396983No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3570.3543072647876141No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3360.333465660976578No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3280.32552600238189755No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.27094084954346964No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2580.2560539896784438No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2310.22925764192139736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2190.21734815402937674No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.19849146486701072No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1960.1945216355696705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1890.18757443429932513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1870.185589519650655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1830.1816196903533148No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA1830.1816196903533148No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1740.17268757443429933No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1740.17268757443429933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1730.17169511710996427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1690.16772528781262405No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1660.1647479158396189No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1650.16375545851528384No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1650.16375545851528384No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC1600.15879317189360856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG1560.15482334259626834No Hit
TCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1550.1538308852719333No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA1540.15283842794759825No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT1520.15085351329892815No Hit
GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.14886859865025803No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1480.14688368400158794No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1460.14489876935291782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT1460.14489876935291782No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA1410.13993648273124257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1350.13398173878523223No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAA1330.1319968241365621No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1330.1319968241365621No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA1320.13100436681222707No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1310.130011909487892No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC1270.1260420801905518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT1250.1240571655418817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1240.12306470821754664No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1240.12306470821754664No Hit
GTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.1220722508932116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1230.1220722508932116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1210.12008733624454149No Hit
GTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.11810242159587137No Hit
GGTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA1180.11710996427153632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1170.11611750694720127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.11611750694720127No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1170.11611750694720127No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAAAAAAAAAAAAAAAAAAA1140.1131401349741961No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA1130.11214767764986106No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA1120.11115522032552601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT1090.10817784835252084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT1080.10718539102818579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1060.10520047637951567No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1060.10520047637951567No Hit
ATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1040.10321556173084558No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGT1040.10321556173084558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1010.1002381897578404No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACC1010.1002381897578404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3100.043.5474345
GCTTGGT301.1326933E-437.49917215
ATGCCGT3650.036.98548544
CACGAGA3950.035.31567421
CAGCAAT3850.035.06416332
GCAATCT3850.035.06416334
AGCAATC3850.035.06416333
GAGACCA3850.035.06416324
AGACCAG3800.034.93343725
ACCAGCA3800.034.93343727
AATCTCG3950.034.74606736
ATCTCGT3950.034.74606737
ACGAGAC3950.034.74606722
CGAGACC3900.034.6146223
CCAGCAG3850.034.4797628
GACCAGC3850.034.4797626
CCACGAG4050.034.44368420
TCTCGTA4000.034.31174538
CGTATGC4000.034.31174541
TCGTATG4000.034.31174540
Produced by FastQC (version 0.11.2)