FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_88.35100000041e24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_88.35100000041e24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36063
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC626317.366830269251032No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT8742.42353658874747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG2500.6932312896874914No Hit
ATACTAACTGTAATCTGGTAAACCCTTTGATTTTAAATGCTTTTGCAAAAT880.24401741396999693No Hit
ACTATAAGGAAGCTACATACGACTATCTATTTAACAGAATCAAACAAATCA760.21074231206499738No Hit
ATTACTAACAGAAACACTGCATAATCATTTCATAAGTTCGTTTGAAAACCA690.19133183595374761No Hit
AGTTAGAATAATCCTAATGACTACTGACTAATGACCATTGACCTTAATTGA590.16360258436624797No Hit
TAGCATACTACTCTGCATGAAGATTTTTCAATTCTTCTATCACTTCGCTAT580.160829659207498No Hit
AAAGTAGACATACAGACTAATGGAACAGAATTGAGAGTCCAGAAATAAATG540.1497379585724981No Hit
GACTTTAGACTGTAAAACTGAAGTGAGATATGACTTCATATCTATAGGGCT520.1441921082549982No Hit
ATATCAGGTGAATGATCTGCGATTGTATTCTCAAAACGATATTCGTTTTCT520.1441921082549982No Hit
ACTCATTTGTTTTGTTTCTTAAGTTCCACATTGGAGTGAAATCATATGGTA510.14141918309624824No Hit
GACAAAGATAAATTCCTAGAAAAGGAAATTGAACGCAATTTAGAGCTCTTC500.13864625793749827No Hit
GCTTTATAGATTTTCTGAATTTCAGCATCATAGAAAGCCTTATCTTTTCCT470.13032748246124837No Hit
TGTGTATTGATGTGTATTGATGGGAATACTCATTCATAGAGGAAGCAGATA440.12200870698499847No Hit
CCTTGAATAATTGGTGTATTATCTCCATCGAAACCATAAGAGCTTAATAAC440.12200870698499847No Hit
GTATTATAATGAAGCGGGTAAACCATTTGATACAAAATTGATTTACGATGA430.11923578182624851No Hit
GAAAAGAATCGAATCAAATTTAATGGAATCGAAAAGAATCGAATTGAATCG430.11923578182624851No Hit
CTATGATATTTACCTCTTTGACACAATCGCTAATGATTTTAAATACCTAAC420.11646285666749853No Hit
AAATACAAGGAAATCTGGAAACTACATCGGAAAAATCAAAGTCACATTTGA420.11646285666749853No Hit
AAATAAGACCTACTATAGTCTCACCCTAATAAAGACTACATTCAAACTACA410.11368993150874859No Hit
CATTTATTGTTCAGCTTATTTACCACCATATCTAAGGCCCTATAGTCGGAT410.11368993150874859No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA400.11091700634999861No Hit
TAGGAGTAACAGCATCTATTATACTTGTGGGTCCTGTAGGAAAGTATTTTG390.10814408119124863No Hit
TAGATAATAAGATGGTAACTACAGAGTGAGATCAGGTTCCTGTTCATCATG390.10814408119124863No Hit
TACTTAACCCGATTTAAATTAAATATCCACCAAACAACTTCAATTCGAAAG390.10814408119124863No Hit
ATTTAAAGGGTCTACCAGATTTGATTAAGTGTGGTTTGGATTTTTAGTTAC390.10814408119124863No Hit
ATTCGATTCACTTGTTTTTTATTCGATTCGATTCAATTCTTTTCATTCCAA380.10537115603249869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7250.044.06081445
ATGCCGT8350.038.25639744
GTATGCC8500.037.58128442
TATGCCG8500.037.58128443
CGTATGC8500.037.58128441
AGCCCAC8550.037.3615117
ATCTCCG8550.037.3615110
GAGCCCA8550.037.3615116
CACGAGA8550.037.3615121
CGAGCCC8550.037.3615115
AGTATCT8500.037.31662834
ACGAGAC8500.037.31662822
TCGTATG8500.037.31662840
TATCTCG8450.037.27121436
CTCGTAT8450.037.27121439
TTAGTAT8650.037.1896532
GCCCACG8600.037.14429518
CTTAGTA8600.037.14429531
CCGAGCC8600.037.14429514
TCTCCGA8600.037.14429511