FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_87.35100000041e0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_87.35100000041e0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119933
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC2008116.743515129280514No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT1858315.494484420468094No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG18581.5491983023854987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAAT207.0105685E-444.99666235
ATAGGGC207.0105685E-444.9966628
AGAAACG207.0105685E-444.99666232
TAGTCCT207.0105685E-444.99666210
TGCCGTC20350.044.22276745
TAAGGTG301.131545E-437.5128635
AAACGTC301.13432936E-437.4972234
ATATAGG301.13432936E-437.497226
AATAACG250.002096071636.012354
AGTCCTT250.002100370435.9973311
ACACCTA250.002100370435.9973313
GAAACGT250.002100370435.9973333
TAGGGCA250.002100370435.997339
AGCTCCC250.002100370435.9973331
GGTAGAT250.002100370435.9973337
GTACAGT401.540933E-533.7756651
AATATAG401.5453461E-533.7615785
TATAGGG352.8066538E-432.1404727
ATAATGT300.005110056630.0228183
CAATACT300.005110056630.0228181
Produced by FastQC (version 0.11.2)