FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_86.35100000041def.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_86.35100000041def.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368729
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC86582.348065923754302No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA44161.19762752590656No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC38861.0538905266469412No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC34540.9367313121560821No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC31690.8594387748183625No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT30080.8157752712696859No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC28960.7854006601053891No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG27130.7357707150780112No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA25930.7032264888305504No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC17590.4770441164106973No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC17220.46700964665106354No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA15730.4266005657271329No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC14920.40463321301009686No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC14880.40354840546851484No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT14360.38944590742794843No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG13390.3631393245445842No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA13210.3582576906074651No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG12980.35202004724336844No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG12860.3487656246186223No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT12460.33791754920280204No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT10640.28855880606081974No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC9240.2505905421054487No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT8940.24245448554358348No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC8870.24055607234581494No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC8670.2351320346379048No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG8270.22428395922208452No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA7850.2128934800354732No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA6940.18821410846448203No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG6720.1822476669857809No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA6020.16326353500809537No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA5890.15973791049795377No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT5840.15838190107097624No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC5750.15594108410241667No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC5730.15539868033162565No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA5720.15512747844623015No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5320.14427940303040987No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5280.14319459548882785No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC5130.1391265672078952No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA5100.1383129615517087No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG4520.1225832521987693No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC4080.11065036924136695No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT4070.11037916735597145No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA4020.10902315792899392No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTA3870.10495512964806132No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAA3780.10251431267950174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC451.922308E-839.999888
TATAGGT451.922308E-839.999883
GGTCGCG504.8637958E-835.999897
TGCCGTC3400.035.73518845
CGTCTTA3550.035.4928528
TTAGTAT3600.034.99989732
TAGTATC3650.034.52044333
TCTTAGT3750.034.19989830
GTCTTAG3750.034.19989829
CGTATGC3700.034.0539541
TCGTATG3700.034.0539540
TATGCCG3650.033.2875743
GTATGCC3700.032.83773842
TATCTCG3850.032.72717736
CTCGTAT3900.032.307639
ATGCCGT3800.031.97358944
GTATCTC4100.031.8291735
AGTATCT4000.031.49990534
GGGGCTA501.9768413E-631.49990536
CGAGACG3950.031.3290223
Produced by FastQC (version 0.11.2)