FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_85.35100000041dc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_85.35100000041dc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620975
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC134832.17126293329039No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT110421.7781714239703692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG13580.2186883529932767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13350.043.47972545
GGGGAAC551.127064E-732.72469738
CCGGGGG551.127064E-732.72469735
GCGTAAG300.005123161230.0266531
GGGTGCC300.00514743829.9976397
ACCGGGG602.4176188E-729.99763934
TCGGGCG406.1657646E-428.12278637
ATCGGGC406.1657646E-428.12278636
CGGGGGA654.8706715E-727.69012636
CCGTTAC1150.027.4156361
CGGGTGG507.129604E-527.0131024
CGTTACT1159.094947E-1225.4471232
TGAGGCG952.1133019E-823.68234626
ATGCCGT24950.023.26470244
TATCCTA602.457653E-422.5109185
AGGGTGC500.002260246522.5054786
GGCGAAC903.2843855E-722.49822829
GGGGGAA802.9731327E-622.49822837
TATGCCG26050.022.36868143
CGTATGC26150.022.2831441
Produced by FastQC (version 0.11.2)