FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_82.35100000041d68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_82.35100000041d68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383264
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC139993.65257368289221No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC47381.2362235952241796No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA22230.5800179510728898No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC21530.5617537780746431No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC18910.4933935877097771No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT17950.46834557902646734No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC16780.43781831844368374No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG16150.42138056274526176No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC15980.416944977874259No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT15240.3976371378475411No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA12100.31570927611254906No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC12080.31518744259831344No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC10190.26587417550304754No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA9910.25856850630374884No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC9650.2517846706186858No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9090.2371733322200885No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA9090.2371733322200885No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA9040.23586874843449945No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT8880.23169408032061453No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA8720.22751941220672958No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT8690.22673666193537612No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC8420.21969190949319528No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG8070.210559822994072No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG7710.20116681973783085No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT7540.19673123486682811No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT7360.19203473323870754No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC7260.18942556566752944No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA7250.18916464891041163No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7090.18498998079652668No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT6990.18238081322534858No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG6970.18185897971111298No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT6870.17924981213993488No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC6840.17846706186858144No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6770.17664064456875678No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA6760.176379727811639No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG6750.17611881105452115No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT6510.16985680888369376No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT6440.1680303915838691No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT6420.16750855806963344No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6420.16750855806963344No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC6150.1604638056274526No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA6050.15785463805627453No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA5930.1547236369708608No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG5920.15446272021374302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.1515926358854471No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5730.14950530182850463No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT5480.1429823829005594No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT5420.14141688235785255No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC5270.1375031310010854No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT5220.13619854721549635No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA5180.13515488018702512No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG5010.13071929531602236No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG4960.12941471153043335No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4850.12654462720213744No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGG4820.125761876930784No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT4740.12367454287384153No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC4700.1226308758453703No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAG4660.12158720881689905No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG4640.12106537530266344No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4630.12080445854554564No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAC4620.12054354178842781No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4500.11741254070301411No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC4500.11741254070301411No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA4220.11010687150371544No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.10984595474659764No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC4190.10932412123236203No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4170.10880228771812642No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA4110.10723678717541955No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4100.10697587041830176No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC4090.10671495366118393No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG4070.10619312014694832No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG4020.10488853636135927No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT3950.1030621190615346No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC3950.1030621190615346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14050.043.55644645
ATGCCGT16000.038.38862244
TATGCCG16100.038.1501843
TATCTCG16200.037.9146936
CGTATGC16250.037.79802741
TCGTATG16250.037.79802740
CGAGACG16350.037.70445323
ATCTCGT16300.037.68208337
CTCGTAT16300.037.68208339
GTATGCC16300.037.68208342
AGTATCT16250.037.65957334
GTCTTAG16250.037.65957329
GTATCTC16350.037.56684535
TCTTAGT16300.037.54405230
CGTCTTA16300.037.54405228
CTTAGTA16250.037.5211231
AGACAAT301.137104E-437.5078286
TTAGTAT16350.037.2916332
CCACGAG16550.037.2488120
AGACGTC16500.037.2253325
Produced by FastQC (version 0.11.2)