FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_80.35100000041d32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_80.35100000041d32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34921
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC6901.9758884338936455No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT2080.5956301365940265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG890.254860971908021No Hit
AAGGAAAGGAAAGGAAAGAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAG460.1317258955929097No Hit
AGAAGAAGTAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAAGCAGCAGAAGA410.1174078634632456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTAC1150.039.13043629
AGCCCAC1100.038.86363617
CCACGAG1100.038.86363620
GAGCCCA1100.038.86363616
CCCACGA1100.038.86363619
ACGAGAC1100.038.86363622
CGAGCCC1100.038.86363615
GACTGAT1100.038.86363626
CCGAGCC1100.038.86363614
CGAGACT1100.038.86363623
TGATATA301.1174027E-437.4999962
TCCGAGC1200.037.49999613
GCCCACG1150.037.17391618
CACGAGA1150.037.17391621
AGACTGA1150.037.17391625
GAGACTG1150.037.17391624
GTATGCC1100.036.8181842
AATCTCG1100.036.8181836
ATCTCGT1100.036.8181837
TATGCCG1100.036.8181843
Produced by FastQC (version 0.11.2)