FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_8.3510000004142e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_8.3510000004142e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35790
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC32098.966191673651858No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT3550.9918971779826767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG1260.3520536462699078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3400.041.0294145
CTCGTAT3850.036.81818439
AGCCCAC3850.036.81818417
CACGAGA3850.036.81818421
TTATCTC3850.036.81818435
CGTTATC3850.036.81818433
TATCTCG3850.036.81818436
TCTCGTA3850.036.81818438
ACAATGC3850.036.81818427
ATCTCGT3900.036.34615337
GCCCACG3900.036.34615318
GACAATG3900.036.34615326
GCGTTAT3900.036.34615332
CCCACGA3900.036.34615319
TCGTATG3900.036.34615340
GAGCCCA3900.036.34615316
ATGCGTT3900.036.34615330
CGAGACA3900.036.34615323
ACGAGAC3900.036.34615322
GTTATCT3900.036.34615334
Produced by FastQC (version 0.11.2)