FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_79.35100000041d0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_79.35100000041d0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268668
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC235438.762859737668796No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT51881.9310077865618533No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG37491.3954025042059344No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA10390.3867226465377343No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA10280.38262837405273425No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9950.370345556597734No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC9530.3547128798368246No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT9140.34019682284455166No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA8550.31823663406136943No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC8350.3107925022704602No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT8050.2996263045840964No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA7820.2910655530245507No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC7610.2832492146440961No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7320.2724552235472777No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7260.2702219840100049No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA7190.2676165378831867No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6880.2560781336072774No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA6560.2441675227418226No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC6080.22630160644364047No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5910.21997409442136764No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACACATCTCC5880.21885747465273125No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5650.21029672309318564No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5640.20992451650364016No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5440.20248038471273097No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5340.19875831881727637No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5180.19280301338454897No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA4780.17791474980273053No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4650.17307606413863952No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4640.17270385754909404No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4610.17158723778045767No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4340.1615376598627302No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT4290.1596766269150029No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4260.15856000714636653No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA4120.15334911489273004No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4100.15260470171363916No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4080.15186028853454822No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4060.15111587535545729No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT4040.15037146217636638No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA3990.14851042922863908No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3970.14776601604954814No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3800.1414385040272753No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3790.14106629743772983No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA3360.125061414087275No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3350.12468920749772953No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3340.12431700090818408No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3340.12431700090818408No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3330.12394479431863863No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC3310.1232003811395477No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT3230.120222728423184No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3200.11910610865454763No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACATCTCC3180.1183616954754567No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3160.11761728229636578No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACATCTCC3130.11650066252772938No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3100.115384042759093No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3060.11389521640091116No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC3050.1135230098113657No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC2980.11091756368454748No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC2960.11017315050545655No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACA2940.10942873732636563No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACAT2910.10831211755772924No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2890.10756770437863833No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATACAC2890.10756770437863833No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2880.10719549778909286No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2880.10719549778909286No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2880.10719549778909286No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT2870.10682329119954741No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2830.10533446484136555No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTCC2810.10459005166227463No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACA2790.1038456384831837No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATAC2790.1038456384831837No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACA2750.10235681212500186No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACAC2720.10124019235636549No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC2700.10049577917727456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24150.044.8103345
GGTGAGT504.8266884E-836.024141
AGGGTGA250.00209924736.017435
TGAGTAA504.83451E-836.017433
ACAGGGT250.00209924736.017433
GTGAGTA504.83451E-836.017432
ATGCCGT30150.035.89285344
ATTCAGC401.5569292E-533.74748645
ATATGTG551.11747795E-732.743125
AATATGT551.11747795E-732.743124
CAATATG551.11747795E-732.743123
TATGTGC551.1193151E-732.7370226
ATACGTC352.806678E-432.158423
CGTCCAC352.8097472E-432.152436
GATATGC352.8158943E-432.14046531
TGTACGG708.094503E-1032.14046520
TATGCCG34150.031.6887143
CGTATGC34150.031.62282841
TCGTATG34150.031.62282840
CTCGTAT34200.031.57659539
Produced by FastQC (version 0.11.2)