FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_78.35100000041cfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_78.35100000041cfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510544
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC189563.7129023159610144No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT105922.074649785327024No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG35040.6863267416716287No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11090.21721927982700806No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9270.18157103011689493No Hit
ATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTA9180.17980820458177943No Hit
GTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTATGTTA9060.1774577705349588No Hit
GATTTAAAGTGATCCAAAATTTGACCCTGTCTCCGAATCTTTGGAATAGAA8740.17118994641010374No Hit
GTCATTGATGGACAATTACGAATTCGGAAATGGTTACACCCTCCGGTTTGG8510.16668494782036417No Hit
TGAGCAATCAGCTTTAGTTAAAGGATCACTTGATTTTCTAGGGTTGAACTA6880.13475821868438373No Hit
ACTTAATGCAATAACGGATGCACGAGTTACTCTTGGATTTTATCGCAATGG6840.13397474066877685No Hit
ATTACGTTTCACAATATGCAACTGACGCACCTCCTCCGACACAACTTAATC6690.13103669811025104No Hit
ACATAGAGCACTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAA6560.12849039455952865No Hit
GTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTG6420.12574822150490458No Hit
CTTATAAGGAGTATCAATGTGGACTATGAAAAGGACGGCCAAGTTGTAACA6280.12300604845028049No Hit
GTATAGTGGAGGACTACACAAACTACGCTGAGCTTCTATTCCAAAGATTCG6260.12261430944247703No Hit
ATTAAGTTGTGTCGGAGGAGGTGCGTCAGTTGCATATTGTGAAACGTAATC6090.1192845278761478No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5990.1173258328371306No Hit
GAGAAAGGCTGATTTAAAGTGATCCAAAATTTGACCCTGTCTCCGAATCTT5930.11615061581372027No Hit
CTTCCACTTATTTTGCGAGGAACTTAGAGAACCATTTTCCAGAAGCTTTTT5900.1155630073020151No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5850.1145836597825065No Hit
GGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAG5770.11301670375129273No Hit
GGACTATTTCACGCATAGATATCCAGAAAAAGTACCGGATCGTAGTTCAGG5740.11242909523958758No Hit
CTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAAAGTACCGGAT5670.11105800871227553No Hit
GTCTTAAATGCGCTATGAAGGATGGATGCAACGTAGCAGGATATTTTGCAT5540.10851170516155317No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5450.10674887962643768No Hit
GTGTAACCATTTCCGAATTCGTAATTGTCCATCAATGACCATGCAAAATAT5440.10655301012253596No Hit
ATCTCCTGAACTACGATCCGGTACTTTTTCTGGATATCTATGCGTGAAATA5420.10616127111473253No Hit
TCAGCAGATCTTGCTTGTGATTCGTATGATCTTTATAAGGATGATGTCAAA5260.10302735905230499No Hit
CATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGT5120.10028518599768091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19750.044.42680445
CTTAATC1200.031.87243545
TAACGCA501.9724557E-631.5128944
GTCTTAT602.401739E-730.0181641
CAATATT300.00512940330.0181641
TCTGCCC300.005146635729.9975858
TACTTAG852.0190782E-1029.1295682
ATGCCGT30550.028.8683844
GTACTTA952.3646862E-1128.4382631
ATAACGC406.146677E-428.1365133
TGTGCAC406.1643147E-428.12273410
TCTTATG1004.5474735E-1127.0110512
TACGGTT755.6306817E-826.9978247
GTACTGG1450.026.395281
GTATAAA1058.367351E-1125.7298551
TATGCCG34350.025.60929343
CGTATGC34550.025.52616341
GTATGCC34700.025.35098342
ACGAGAC35300.025.11375222
TTACAAT35050.025.09783732
Produced by FastQC (version 0.11.2)