FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_77.35100000041cd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_77.35100000041cd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255610
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC206578.081452212354758No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT41651.6294354680959275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG24860.9725754078478933No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC15890.6216501701811353No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA9410.36813896169946403No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG8520.3333202926333086No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA8310.32510465161769886No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT8260.32314854661398223No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7960.31141191659168266No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC7810.30554360158053284No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG7760.3035874965768163No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC7100.27776691052775715No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6920.27072493251437735No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA6770.2648566175032276No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6320.2472516724697782No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC6010.23512382144673527No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT6000.23473260044599192No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT5950.23277649544227536No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5610.21947498141700245No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT5530.21634521341105592No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC5300.20734713039395955No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5280.20656468839247288No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAG5170.2022612573842964No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG4870.1905246273619968No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4780.18700363835530692No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4670.1827002073471304No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA4440.17370212433003404No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA4260.1666601463166543No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC4190.16392159931145103No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA4110.16079183130550448No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT4110.16079183130550448No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA3630.14201322326982513No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT3620.1416220022690818No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA3490.13653612925941866No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG3450.13497124525644535No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGTCTCTTATA3430.13418880325495872No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG3210.1255819412386057No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3140.12284339423340246No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3140.12284339423340246No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3110.1216697312311725No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT3070.12010484722819921No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT3040.11893118422596925No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA3030.11853996322522595No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCTGTCTCTTATA3010.1177575212237393No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2980.11658385822150934No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2980.11658385822150934No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA2950.11541019521927937No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG2890.11306286921481945No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2850.11149798521184616No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2850.11149798521184616No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACAT2820.1103243222096162No Hit
GTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCAC2730.10680333320292634No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA2620.1024999021947498No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2620.1024999021947498No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2600.10171746019326318No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA2580.10093501819177654No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA2580.10093501819177654No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA2560.10015257619028989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG406.7684596E-945.0076264
GTTAGCC406.7684596E-945.0076263
CGGGGAT207.01712E-445.0076264
AGTTAGC406.7684596E-945.0076262
GAGTTAG406.7684596E-945.0076261
ATACGTC207.01712E-445.0076263
GGCGTAA207.023888E-444.99882538
CTGGGCG207.023888E-444.99882535
TGCCGTC20750.044.565145
TAGCCGG403.4395816E-739.381675
GCCGGTG403.444875E-739.3739747
GGTGAGT1000.038.2564851
TACGCCC301.13630456E-437.506364
TTACGCC301.13630456E-437.506363
ATGCCGT25050.036.91520744
TGAGTAA1050.036.434753
ACGTCCA250.002102300336.0061045
TATACGT250.002102300336.0061042
CGTCCAC250.002102300336.0061046
TACGTCC250.002102300336.0061044
Produced by FastQC (version 0.11.2)