FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_74.35100000041c76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_74.35100000041c76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257813
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC76732.976188167392645No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC22830.8855255553443775No Hit
GGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAG17050.6613320507499622No Hit
ACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCA16200.6283624177213717No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT15780.6120715402248917No Hit
TACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCATAT13910.5395383475619926No Hit
TAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTG13800.5352716891700574No Hit
GTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAG12100.46933242311287643No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11270.43713854615554687No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC9400.3646053534926478No Hit
AATACATACAGTGCAATTGTTATTACATTCGAGGTTTGGATTTATATCTAG8900.345211451711124No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC8820.34210842742608016No Hit
ACAATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAATTCACACCTGAG8580.3327993545709487No Hit
ATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTA7540.2924600388653792No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC7430.28819338047344395No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT7380.2862539902952915No Hit
GAATGGGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGG7330.28431460011713916No Hit
GTACAATTGTTATTACATTCGAGGTTTGGATTTATAAATCTAGGGCTTCAA7060.2738418931551163No Hit
TTGTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAA7020.2722903810125944No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG6980.2707388688700725No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA6280.24358740637593917No Hit
GTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTG5640.21876321209558866No Hit
GTTGCATATTGTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCT5310.20596323691978294No Hit
CAGTACAATTGTTATTACATTCGAGGTTTGGATTTATAAATCTAGGGCTTC4970.19277538370834676No Hit
CAACAATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAATTCACACCTG4900.1900602374589334No Hit
ACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTGAT4830.1873450912095201No Hit
ACGTAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTGAT4670.18113904263943245No Hit
TAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGT4660.180751164603802No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC4630.17958753049691054No Hit
TGAGCAATCAGCTTTAGTTAAAGGATCACTTGATTTTCTAGGGTTGAACTA4340.16833906746362673No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA4220.16368453103606104No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC4200.16290877496480008No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC4090.1586421165728648No Hit
GAAATATAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGA3990.15476333621656005No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC3780.14661789746832005No Hit
GAACCATATGTTACTATATTTCATTGGGATGTTCCCCAAACTTTAGAAGAC3770.1462300194326896No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA3700.14351487318327627No Hit
AAACGTAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTG3690.14312699514764576No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG3690.14312699514764576No Hit
GTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCT3460.13420580032814483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG3430.1330421662212534No Hit
CTATACGTGTGCTTAAGAAACCTCCATATTCGTCTTCTAAAGTTTGGGGAA3360.13032701997184004No Hit
GCATAGAACCATATGTTACTATATTTCATTGGGATGTTCCCCAAACTTTAG3340.12955126390057908No Hit
TCAGCAGATCTTGCTTGTGATTCGTATGATCTTTATAAGGATGATGTCAAA3260.12644823961553528No Hit
GAGTCTGTAAGCTTGAACATTCATTCTTTTTAGCAGTTTGACATCATCCTT3260.12644823961553528No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT3230.12528460550864387No Hit
ATATAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGAT3190.12373309336612195No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT3130.1214058251523391No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG3120.12101794711670863No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG3070.11907855693855623No Hit
ACATAGAGCACTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAA3030.11752704479603432No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT2960.114811898546621No Hit
CTTTTTAGTAGTTTGACATCATCCTTATAAAGATCATACGAATCACAAGCA2650.10278767944207623No Hit
CTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCATATGT2650.10278767944207623No Hit
CCACTATACGTGTGCTTAAGAAACCTCCATATTCGTCTTCTAAAGTTTGGG2650.10278767944207623No Hit
GAAAATATCCAACGATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAAT2620.1016240453351848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8700.043.18723745
AGTGTCT301.1378425E-437.49799
CGTATGC10350.036.9544541
TATGCCG10200.036.8361743
AATCTCG10500.036.64791536
CTCGTAT10500.036.6408139
GTATGCC10300.036.4785442
ATGCCGT10300.036.4785444
TTACAAT10550.036.47422832
GACTGAT10500.036.43359826
ATCTCGT10600.036.30217737
TGATTAC10550.036.26092529
CACGAGA10600.036.08288621
CGTGATA250.00210466335.99798625
TCTCGTA10700.035.96290638
CTGATTA10650.035.9204528
GATTACA10650.035.9204530
CGAGACT10750.035.78869223
CCACGAG10700.035.7456620
CCCACGA10850.035.45884319
Produced by FastQC (version 0.11.2)