FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_72.35100000041c26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_72.35100000041c26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45947
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC14213.0926937558491305No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT7181.5626700328639518No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG1730.3765207739351862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.040.78125445
ATACTAG250.002086147436.01
ATGCCGT2100.031.07142844
TACAAAG554.051646E-628.6363642
ATCTCGT2300.028.36956437
GCCCACG2300.028.36956418
CCCACGA2300.028.36956419
TCTCGTA2300.028.36956438
CGTATGC2300.028.36956441
CTCGTAT2300.028.36956439
TCCGAGC2400.028.12500213
CCACGAG2350.027.76595720
GAGCCCA2350.027.76595716
CACGAGA2350.027.76595721
ACGAGAC2350.027.76595722
TATCTCG2350.027.76595736
CGAGCCC2350.027.76595715
TCGTATG2350.027.76595740
CGAGACT2350.027.76595723
AGACTCA2350.027.76595725
Produced by FastQC (version 0.11.2)