FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_71.35100000041c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_71.35100000041c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276025
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC4914817.805633547685897No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT119294.321709990037134No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG87673.176161579567068No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC13860.5021284303957975No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA12960.46952268816230414No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA12540.4543066751200073No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC12040.43619237387917764No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA11910.43148265555656196No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10480.3796757540077892No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA10280.3724300335114573No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9780.3543157322706276No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9350.3387374332035142No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA9110.330042568607916No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC8040.2912779639525405No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7600.27533737886061044No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA7230.2619327959423965No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA6630.24019563445340097No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC5800.21012589439362378No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5570.20179331582284213No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5570.20179331582284213No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCCTGTCTCTTATAC5200.1883887329046282No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5110.18512815868127885No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC5100.18476587265646227No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTA4730.17136128973824835No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC4320.15650756272076805No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATACAC4200.15216013042296894No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC4180.15143555837333575No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATAC3990.14455212390182048No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3940.14274069377773752No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACAC3930.14237840775292093No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3860.13984240557920477No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA3830.138755547504755No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTT3740.13549497328140567No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA3650.13223439905805634No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATA3610.13078525495878998No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3570.1293361108595236No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3400.12317724843764152No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3210.11629381396612627No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAC3150.11412009781722669No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC3140.1137578117924101No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA3130.11339552576759351No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTT3120.11303323974277693No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACT3100.11230866769314375No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTTA3090.11194638166832714No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3090.11194638166832714No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT2990.10832352142016123No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG2930.10614980527126165No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG2890.1047006611719953No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACA2890.1047006611719953No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2860.1036138030975455No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT2860.1036138030975455No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCCTGTCTCTTATA2810.10180237297346255No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT2800.10144008694864595No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCCTGTCTCTTATA2790.10107780092382937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC50150.044.7724345
CGGGTAT301.1345232E-437.5176774
GCCGTCT301.1381609E-437.49728445
ATGCCGT61750.036.39817444
GACGTAT250.002105089835.99739528
ACGTATA250.002105089835.99739529
ATTTTTG1500.034.497513
TATGTGC850.034.4279826
CCCTGTA1050.034.2832335
TGTACGG1450.034.13545620
TGTCACC1400.033.74755530
TTGTGTA1500.032.9976117
GTCACCC1450.032.58384731
CATTTTT1600.032.34140812
GGATCCT352.8070883E-432.1580056
CGGGATC352.8070883E-432.1580054
GGGATCC352.8070883E-432.1580055
CGGGTGT352.81606E-432.1405317
TGTGTAC1550.031.93316818
CTGTCAC1500.031.49771929
Produced by FastQC (version 0.11.2)