FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_70.35100000041bfe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_70.35100000041bfe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374062
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC104422.791515844966877No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT59821.5992001325983392No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG24210.6472189102341324No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9770.2611866482027044No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6770.18098603974742156No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4400.11762755906774812No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA4150.1109441750298079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC207.027795E-444.999237
TGCCGTC11300.044.40186345
CCCTACC300.005143321629.99946622
GATGGCG300.005143321629.99946633
ATGCCGT18700.026.83107244
GGGGAAC3300.025.22682438
ACGGGTA1001.3387762E-924.74955715
ACGGGAC651.5203934E-524.23033515
ACCGGGG3350.024.17867334
CGGGTAT851.8868741E-723.82310716
AACCGGG3400.023.82310733
CCGGGGG3400.023.82310735
TATGCCG21550.023.28264843
GTATGCC21650.023.17510442
GAACCGG3500.023.14244532
CGGGGGA3600.023.12458836
CGTATGC21900.023.0132941
CTCGTAT22000.022.80641239
TCGTATG22000.022.80641240
TATCTCG22300.022.60049436
Produced by FastQC (version 0.11.2)