FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_7.35100000041404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_7.35100000041404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406931
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC181404.457758194878247No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT32960.8099653258169075No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC30180.7416490756418165No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA17080.4197271773347324No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC13880.3410897670612463No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC13410.329539897427328No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG12440.3057029324381775No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT11370.2794085483779805No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10840.2663842273014344No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA8450.2076519115034244No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC8190.20126262191870364No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8040.19757649331213398No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC7360.18086604362901817No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC7260.17840862455797174No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC7120.1749682378585067No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT7080.17398527023008817No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC6910.1698076578093092No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG6780.16661301301694884No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6380.15678333673276307No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA6270.15408017575461197No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT6110.15014830524093767No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA6020.14793662807699584No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5510.135403790814659No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA5460.13417508127913577No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT5350.13147192030098467No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC5270.12950598504414754No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA5070.12459114690205465No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG4960.12188798592390356No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG4950.1216422440167989No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT4920.12090501829548497No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG4910.12065927638838035No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT4910.12065927638838035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.11943056685285712No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT4700.1154986963391828No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG4600.11304127726813637No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT4530.11132108391840385No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC4440.10910940675446207No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG4310.10591476196210169No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4290.10542327814789239No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA4210.10345734289105525No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4140.10173714954132274No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT4070.10001695619159022No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG253.8878534E-544.9970514
ATTGTCG207.0301787E-444.997058
CGCTGCA351.2099736E-744.9970529
TATGCGA207.0301787E-444.9970510
TCAGGAC253.8878534E-544.9970527
GTAGCGA253.8878534E-544.9970520
AGTGCCC207.0301787E-444.9970510
AGTGACG351.2099736E-744.9970537
GACGAAT302.1630094E-644.9970540
TGCCGTC18950.044.40342745
AATGACG403.4526602E-739.37241735
CGTAACC403.4526602E-739.37241719
TCTAATC356.2411527E-638.568921
CGAATTT356.2411527E-638.568942
ACCCAAT356.2411527E-638.568917
CTAATCG356.2411527E-638.568935
TGCGTAT356.2411527E-638.568941
ACGAATT356.2411527E-638.568941
ATGCCGT22350.037.64853744
GTTAGGT301.13906586E-437.49754345
Produced by FastQC (version 0.11.2)