FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_68.35100000041bae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_68.35100000041bae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172140
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC165349.604972696642267No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT32841.907749506215871No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG21741.2629255257348668No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC13390.7778552341117695No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9900.5751132798884628No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8220.4775182990589055No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7150.4153595910305565No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT5970.34681073544789126No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT5950.34564889043801555No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT5540.3218310677355641No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA4630.2689671197862205No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT4600.267224352271407No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG4160.24166376205414197No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3900.2265597769257581No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA3860.22423608690600674No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA3510.2039037992331823No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3100.1800859765307308No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC3060.17776228651097944No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG2940.17079121645172535No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA2840.16498199140234693No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT2680.15568723132334147No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT2670.15510630881840362No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2670.15510630881840362No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT2550.14813523875914952No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2530.14697339374927385No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2520.14639247124433602No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA2500.14523062623446034No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC2480.14406878122458466No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA2400.13942140118508192No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT2400.13942140118508192No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2260.13128848611595215No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT2260.13128848611595215No Hit
GGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATAC2230.12954571860113862No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC2200.12780295108632508No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2140.12431741605669804No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT2060.1196700360171953No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT2030.11792726850238179No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC2010.1167654234925061No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT1970.11444173347275473No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC1950.11327988846287905No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC1940.11269896595794121No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT1830.10630881840362495No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA1830.10630881840362495No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA1820.10572789589868711No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCCTGTCTCTTATAC1810.10514697339374927No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCA1740.10108051585918439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17150.044.7370345
ATGCCGT20300.037.7950844
GTATTCC301.13209695E-437.5213171
TCTACGC301.13597955E-437.49951616
CTACGCA301.13597955E-437.49951617
GGCGTAG250.002096286336.0204661
TCACCGC250.00210227835.99953526
ACCGCTA250.00210227835.99953528
CGGGGTC250.00210227835.99953517
GGGGTCT250.00210227835.99953518
CGCTACA250.00210227835.99953530
CACCGCT250.00210227835.99953527
GCGTAGT250.00210227835.9995352
GTAGTCG250.00210227835.9995354
TTAGCCC401.5530362E-533.74956533
TATGCCG22950.033.43094343
CGTATGC23000.033.35826541
CTCGTAT22950.033.33290539
TCGTATG22950.033.33290540
GTATCTC23000.033.2604435
Produced by FastQC (version 0.11.2)