FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_67.35100000041b84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_67.35100000041b84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158247
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC30301.914728241293674No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT19901.2575277888364391No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10530.66541545811295No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT8600.5434542203011747No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8220.5194411268460065No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5330.3368152318843327No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4020.25403325181520026No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3760.2376032405037694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG3710.23444362294387888No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3670.23191592889596643No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT3640.2300201583600321No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3610.22812438782409777No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3230.20411129436892958No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3160.19968782978508284No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT2910.18388974198563007No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT2900.18325781847365197No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC2880.18199397144969573No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG2780.17567473632991462No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2780.17567473632991462No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA2700.17061934823408975No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT2610.16493203662628675No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC2310.14597433126694345No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC2260.1428147137070529No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2050.12954431995551258No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA1990.12575277888364392No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTA1950.12322508483573148No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCC1840.11627392620397227No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC1830.11564200269199415No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA1820.11501007918001604No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC1710.10805892054825683No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC1690.10679507352430062No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC1680.10616315001232252No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA1600.10110776191649762No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1590.10047583840451951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2700.044.9946345
GTCGACT403.4362893E-739.37029634
TGTCGAC403.4362893E-739.37029633
GTCTGCG250.002102863735.99570521
CGTCTGC250.002102863735.99570520
CGTTAAC250.002102863735.99570539
GTATAAA300.00511321930.0248871
GACCAGG300.005137004429.99641834
ACCAGTG300.005137004429.99641831
TCGTCTG300.005137004429.99641819
CGTTTAG300.005137004429.99641826
CTAGGCT300.005137004429.99641845
TAACCTA8200.028.2856435
TCCCCCG654.833819E-727.68900117
ACCAAGT507.125895E-526.9967778
ATAGTGC507.125895E-526.99677727
ATGCCGT4600.026.40989144
CGGTTCG608.140885E-626.24686622
CCCCCGG608.140885E-626.24686618
CCGGTTC608.140885E-626.24686621
Produced by FastQC (version 0.11.2)