H23LNBCXX_l02n01_bir_1228_pa_66.35100000041b6a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pa_66.35100000041b6a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20281
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC	1686	8.313199546373452	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT	583	2.8746117055372022	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	182	0.8973916473546669	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	84	0.41418076031753853	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	78	0.3845964202948573	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	70	0.34515063359794884	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	65	0.3204970169123811	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	41	0.2021596568216557	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	40	0.19722893348454218	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	35	0.17257531679897442	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA	34	0.16764459346186086	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC	31	0.1528524234505202	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG	29	0.14299097677629308	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	28	0.13806025343917952	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	26	0.1281988067649524	No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC	24	0.11833736009072532	No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG	24	0.11833736009072532	No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG	23	0.11340663675361176	No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG	22	0.1084759134164982	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT	22	0.1084759134164982	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG	22	0.1084759134164982	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA	21	0.10354519007938463	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	225	0.0	44.99753	45
CTGAAAC	20	6.872075E-4	44.997528	44
AACCTAT	45	1.7736966E-8	39.997803	6
TAACCTA	45	1.7736966E-8	39.997803	5
ACCTATG	45	1.7736966E-8	39.997803	7
GGGGGAA	35	5.952137E-6	38.569313	37
ACCGGGG	30	1.1011699E-4	37.49794	34
CGGGGGA	30	1.1011699E-4	37.49794	36
AGGCGAA	30	1.1011699E-4	37.49794	28
TAATGAG	30	1.1011699E-4	37.49794	23
GGTTAAT	30	1.1011699E-4	37.49794	20
CGAACCG	30	1.1011699E-4	37.49794	31
CCGGGGG	30	1.1011699E-4	37.49794	35
GGGGAAC	30	1.1011699E-4	37.49794	38
ATTAACC	55	2.4774636E-9	36.816162	3
TTAACCT	55	2.4774636E-9	36.816162	4
GTGTCGA	50	4.4896296E-8	35.998024	32
CCTATGG	50	4.4896296E-8	35.998024	8
GAAACAC	50	4.4896296E-8	35.998024	37
TCGAAAC	50	4.4896296E-8	35.998024	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content