FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_63.35100000041b0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_63.35100000041b0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212358
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC3025114.245283907363978No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT85244.013976398346189No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG58842.7707927179574114No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3190.15021802804697726No Hit
TACCACAAGGCTTGCATTACGTTCCTGTCTCTTATACACATCTCCGAGCCC3140.14786351350078641No Hit
TACCACAAGGCTTGCATTACGTTCTGCAACAAATGCCTGTCTCTTATACAC3010.14174177568069016No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG2960.13938726113449929No Hit
GAACGTAATGCAAGCCTTGTGGTACTGTCTCTTATACACATCTCCGAGCCC2520.11866753312801967No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2220.10454044585087446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29600.044.46511545
ACGTTCC458.659845E-734.99780319
TACGTTC900.034.99780318
ATGCCGT38800.033.9218444
GTATTAG401.5477637E-533.771741
TGGACTA401.5502636E-533.7637835
CATTACG950.033.1558115
TTACGTT950.033.1558117
TATTAGC352.805957E-432.1559832
GGACTAT352.8098398E-432.1484076
CTATGCC352.813727E-432.140849
ATTACGT1000.031.4980216
CGTTCTG501.9725449E-631.4980220
CGTTCCT501.9725449E-631.4980220
AGGCTTG1000.031.498028
GTATAAC300.005121766630.0193231
TATAAGA300.005127672630.012252
GGGATCC300.005127672630.012255
GCAAGCC300.00513950129.99811710
GCATTTT602.404613E-729.99811711
Produced by FastQC (version 0.11.2)