FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_61.35100000041ad5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_61.35100000041ad5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324084
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC307549.489515063995754No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT100073.0877797114328387No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG60081.853840362375187No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC20510.6328606163834067No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA15320.47271694992656227No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT12190.3761370508880414No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10590.326767134446625No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA9420.2906653830488392No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT9400.2900482590933215No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT8910.2749287221831377No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT8560.26412905296157785No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8430.2601177472507128No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT7910.24407252440725244No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA7300.2252502437639624No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA6280.19377692203255945No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA6250.1928512360992829No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG6020.18575431061082928No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6010.18544574863307042No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5990.18482862467755273No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA5900.18205156687772306No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA5900.18205156687772306No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5600.1727947075449575No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA5540.17094333567840436No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT5520.17032621172288667No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5490.1694005257896101No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5420.16724059194529814No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5360.16538922007874501No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5150.15890941854580912No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA5130.1582922945902914No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4870.15026968316856124No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4840.1493439972352847No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4760.14687550141321387No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4740.14625837745769615No Hit
GGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATAC4650.14348131965786648No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT4560.1407042618580368No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4550.14039569988027795No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC4470.13792720405820713No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT4350.1342244603251009No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC4350.1342244603251009No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4300.13268165043630661No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4290.13237308845854778No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCTGTCTCTTATA4280.13206452648078892No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4140.12774465879216498No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG4070.12558472494785303No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT4020.12404191505905877No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT3960.12219054319250564No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3880.11972204737043482No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA3790.11694498957060516No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3790.11694498957060516No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT3790.11694498957060516No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA3770.11632786561508746No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT3760.1160193036373286No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA3750.11571074165956975No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCCTGTCTCTTATAC3620.11169943594870466No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3610.1113908739709458No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT3560.10984806408215154No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC3560.10984806408215154No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3530.10892237814887498No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCCT3440.10614532034904532No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACACATCTCC3370.10398538650473334No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT3350.10336826254921563No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3310.10213401463818023No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3310.10213401463818023No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3280.10120832870490366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32000.044.64598545
TATGTGC1100.036.8275266
GTATAAC250.002099076836.0202561
GTCTATA250.002100668436.0146942
ATTGAAT250.002100668436.0146943
CTATACC250.002102260736.0091364
TAACGCA250.002102260736.0091364
CCCTGCA250.002105448435.9980249
TATTGCT250.002105448435.99802436
AACGCCC250.002105448435.99802420
CCTGCAA250.002105448435.99802410
ATGCCGT41900.034.0434844
CAATATG1250.032.4132273
ATATGTG1250.032.4082265
GCCGTCT352.8168454E-432.1410945
AATATGT1300.031.1617534
TGTACGG1300.031.15213820
CACAATA1350.030.016881
GGCGTAG300.00512774230.0168781
GGTCTAT300.00512774230.0168781
Produced by FastQC (version 0.11.2)