FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_60.35100000041aaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_60.35100000041aaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356231
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC4579512.855422464636709No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT109793.081988934146663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG31590.8867841372592503No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA15950.44774317788176776No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT11190.3141220163320991No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC9610.26976877363283935No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT6940.19481740780560927No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA6380.17909727115270707No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5720.16056996724035807No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA5480.1538327658176857No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4850.13614761208317075No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA4730.13277901137183457No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT4410.12379607614160473No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA4380.1229539259637707No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4230.11874317507460047No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT3830.1075145060368132No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3790.10639163913303447No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA3770.10583020568114511No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG3610.10133873806603018No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT3590.10077730461414082No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3590.10077730461414082No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTC207.026076E-445.0008429
TGCCGTC49550.044.4105245
ATGCCGT59800.036.83080344
TATGCCG62550.035.21154443
AATCTCG62750.035.17595736
TCGTATG63000.035.1742940
CGTATGC62950.035.16649241
ATCTCGT63050.035.15133337
CTCGTAT63000.035.14351339
GTATGCC63000.034.9957442
CGAGACC63250.034.9334623
TCTCGTA63450.034.9297338
ATTAAAT62950.034.92122332
CACGAGA63550.034.87476721
CTGATTA63050.034.86583729
GATTAAA63200.034.8542931
TTAAATC63150.034.8462633
GACCTGA63150.034.8462626
CCTGATT63200.034.81868728
ACGAGAC63850.034.74614722
Produced by FastQC (version 0.11.2)