FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_59.35100000041a85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_59.35100000041a85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239816
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC62462.60449678086533No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT53542.2325449511291993No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT15960.6655102245054543No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA12580.5245688361076826No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT9850.41073156086332857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG8870.3698668979550989No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6620.2760449678086533No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4950.20640824632218036No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA3770.1572038562898222No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3760.1567868699336158No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3550.14803015645328085No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3420.14260933382259733No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3340.13927344297294594No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3260.1359375521232945No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3160.13176768856123028No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT3090.1288487840677853No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3050.1271808386429596No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT2970.1238449477933082No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC2950.12301097508089535No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC2790.11633919338159256No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2750.11467124795676686No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2650.11050138439470261No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC2650.11050138439470261No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2450.10216165727057411No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCC2420.10091069820195485No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGTCTCTTATA2410.1004937118457484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7100.044.68144245
TTAGGTC250.00210417535.9986658
GTCTGCT250.00210417535.99866511
TGTCCCC900.034.99870345
GTCCCAG300.00512445730.0176641
GGCTTTC300.005140165329.99888828
CTCTCGT300.005140165329.9988889
TATAATA554.1413605E-628.6412752
TATTACG406.145639E-428.1298242
TGATAGC507.137E-526.99899925
GCTACCG507.137E-526.9989997
GGCGAAC3050.026.55639329
GCGAACC3100.026.12806330
CAGGTTA957.348717E-1026.0516669
CGAACCG2850.026.05166631
GAACCGG2850.026.05166632
AACCGGG2900.025.602533
ACCGGGG2900.025.602534
TAACCTA9200.025.4391465
CTGTCCC1950.025.38367544
Produced by FastQC (version 0.11.2)