FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_57.35100000041a42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_57.35100000041a42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285684
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC30281.0599123507091752No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT11450.40079248400330436No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10660.37313955279259603No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4830.16906792119964717No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4370.15296621441872837No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3810.13336413659847943No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3670.1284636171434172No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT3520.12321306058442195No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3490.1221629492726229No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3080.1078114280113692No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3080.1078114280113692No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3070.10746139090743619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3400.038.3823545
GCTGTAA250.002104464536.01
ATGCCGT3750.034.844
GGGGTAC352.8151082E-432.1428575
CGTATGC4200.031.07142841
TATGCCG4300.030.34883743
CGACTGT300.005140861529.99999836
GCATGGG300.005140861529.99999811
CTCGTAT4300.029.8255839
GTATGCC4400.029.6590942
TCTCGTA4350.029.4827638
CGAGACC4350.029.4827623
TCGTATG4450.028.82022540
CACGAGA4500.028.521
CCCACGA4600.028.36956619
CCTGATT4600.028.36956628
ATCTCGT4550.028.18681137
GACCTGA4550.028.18681126
GGTAGCC406.154715E-428.1258
ACGAGAC4650.028.06451622
Produced by FastQC (version 0.11.2)