FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_55.35100000041a0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_55.35100000041a0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131101
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC2664920.32707607112074No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT71615.462200898543871No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG56054.275329707629996No Hit
ATCAACTCCTGAGTCTCCTTTATCTTCACCATTTACAGGCTTCCCCTGTTT2590.19755760825622992No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2180.16628401003806226No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA1940.14797751352011043No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT1880.14340088939062248No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT1840.14034980663763053No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1690.12890824631391065No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1590.12128053943143073No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT1550.11822945667843876No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1530.11670391530194278No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1450.11060174979595884No Hit
CTTTCACATTGTTGGCGATAGCTAGTGGTCCATAGCAGTTCAGTTCGTTGT1390.10602512566647089No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1340.10221127222523094No Hit
AAGGAATGTGACAATAACGCAAGAAACAACGAACTGAACTGCTATGGACCA1320.10068573084873494No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGA207.0104166E-445.02
ACGCAAG351.2009514E-745.017
GCGATAG207.0104166E-445.015
ATAACGC351.2009514E-745.014
GTTGGCG207.0104166E-445.011
TGCCGTC26450.044.74480445
GGGGCTG650.041.5384625
AATATGT556.002665E-1140.9090924
CGGGTGT403.427067E-739.37517
CTGAGTC403.427067E-739.3759
GACAATA403.427067E-739.37510
TAACGCA403.427067E-739.37515
CGCAAGA403.427067E-739.37518
CCTGAGT403.427067E-739.3758
GCTGTCA700.038.57142628
TGTACGG700.038.57142620
ATATGTG601.5279511E-1037.5000045
GGGTGTG250.00210032136.018
GGTGTGG250.00210032136.019
CAGTTCG250.00210032136.041
Produced by FastQC (version 0.11.2)