FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_54.351000000419e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_54.351000000419e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149716
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC14880.9938817494456169No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7930.529669507601058No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT7220.4822463864917578No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7150.4775708675091506No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3810.2544818189104705No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3460.23110422399743513No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT1960.13091453151299795No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC1920.12824280638007962No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT1720.11488418071548799No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1690.11288038686579924No Hit
GTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACATCT1520.10152555505089636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.042.18718745
CCATCAG352.8088712E-432.1426234
CCGGGGG1300.031.15361635
GAACCGG1300.031.15361632
ACCGGGG1350.029.99977934
CTACCGT300.005133321529.9997778
GCGAACC1450.029.4825430
AGGCGAA1450.029.4825428
GGCGAAC1450.029.4825429
AACCGGG1400.028.92835633
CGAACCG1400.028.92835631
GGGCGGG406.141187E-428.12479223
GCGGGAC406.141187E-428.12479225
TAGGGCG406.141187E-428.12479221
GGCGGGA406.141187E-428.12479224
CTGAAAC1450.027.93082644
CGGGGGA1500.026.999836
GGGGAAC1550.026.12883838
ATGCCGT2600.025.96134844
ATGAGGC1650.025.90890125
Produced by FastQC (version 0.11.2)