FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_53.351000000419c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_53.351000000419c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152243
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC3041219.97595948582201No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT1796611.800870976005465No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG22861.5015468691499774No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3710.24368936502827715No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3620.23777776318123No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3520.23120931668451097No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA2110.13859422108077218No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2030.13333946388339693No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1920.12611417273700598No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1750.11494781369258357No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA1670.10969305649520833No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1620.10640883324684877No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG1620.10640883324684877No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT1580.10378145464816117No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1560.10246776534881737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG206.984001E-445.039135
TGCCGTC31700.044.1431145
ACTATAC301.1360558E-437.495628
CTATACT250.00210258735.9957969
ACAGAGT250.00210258735.9957968
GCCGTCT606.361006E-933.74605645
TAATACA352.7946665E-432.1708074
TGTGAGC300.00512808530.0063536
AGACAGA300.00512808530.0063536
CCTGTAT300.005136335329.99649436
TGTACGG300.005136335329.99649420
CCCTGTA300.005136335329.99649435
CGGGGCT406.145594E-428.12171424
ATGCCGT51250.027.30412744
TATCTCG53800.026.00997236
TATGCCG53900.026.00345443
TCTCGTA53950.025.97935738
CGAGACG54050.025.97291223
ATCTCGT53900.025.96171637
GAGACGA53850.025.94404624
Produced by FastQC (version 0.11.2)