FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_52.351000000419a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_52.351000000419a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304253
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC43931.4438641525309528No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT11750.3861917548882016No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8820.28989032154161176No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT8490.27904408502134737No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7980.26228171949002965No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT6760.2221835117484462No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT6670.21922544724291954No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC6540.21495268740160328No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC6390.2100225798923922No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT6060.1991763433721278No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA5160.1695956983168613No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5050.16598028614343985No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT5000.1643369169737028No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT4830.1587494617965969No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC4740.15579139729107028No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT4380.14395913926896364No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA4160.13672831492212073No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3790.12456738306606673No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3730.12259534006238229No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG3530.11602186338343418No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3400.1117491035421179No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT3320.10911971287053865No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA3170.10418960536132757No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3130.10287491002553796No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3090.10156021468974834No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3090.10156021468974834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5000.042.74859645
ATGCCGT5700.037.49876844
CGGCCGT301.13817005E-437.49876445
CGTATGC5800.036.85223841
TATGCCG5800.036.85223843
GTATGCC5800.036.85223842
CCCACGA5900.036.60896719
CGAGACG5850.036.53726223
TATCTCG5800.036.46431736
CGATCGT5800.036.46431728
TCGTGTA5750.036.3901131
CACGAGA5900.036.22762321
ACGATCG5850.036.15265727
GAGACGA5850.036.15265724
AAGCTAC250.002105019335.99881727
GCCCACG5950.035.92318718
ATCGTGT5850.035.76805530
GTATCTC5850.035.76805535
ATCTCGT6000.035.62382537
CTCGTAT6000.035.62382539
Produced by FastQC (version 0.11.2)