FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_49.35100000041943.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_49.35100000041943.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297288
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC41281.388552514733188No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC27640.9297381663572024No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA13700.46083259331019083No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC12380.4164312047576761No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10880.36597508140254564No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10760.3619385915341352No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10700.35992034659993005No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG10130.34074701972498045No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT10010.33671052985657No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT9820.33031942089825356No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA7820.2630445897580797No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT6680.2246979360081806No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC6620.2226796910739754No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5870.1974516293964102No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT5770.1940878878394015No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC5440.18298754070127282No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA5410.18197841823417024No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC5190.1745781868087511No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG5150.17323269018594764No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC4660.15675035655660505No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT4570.15372298915529722No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA4560.15338661499959635No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG4450.1496864992868868No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT4390.14766825435268158No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG4320.1453136352627755No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC4150.13959527461586071No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3760.1264766825435268No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3640.12244019267511638No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT3350.11268534215979119No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACA3300.11100347138128684No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3300.11100347138128684No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3260.10965797475848335No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3240.10898522644708163No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA3170.10663060735717554No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGAT3160.10629423320147467No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC3080.1036032399558677No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT3060.10293049164446597No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA3030.10192136917736337No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA2990.1005758725545599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4500.044.49717345
CGGGCAA301.13493435E-437.516554
TCTAACG301.13831265E-437.4976228
ATGCCGT5400.037.0809844
CGACTGT552.73576E-936.8158436
TCTGGCT250.00210525835.99771527
AGACGAT5750.034.82387525
TCGTGTA5750.034.82387531
CGAGACG5750.034.82387523
GAGACGA5850.034.61318624
GATCGTG5800.034.5236729
CACGAGA5800.034.5236721
ATCGTGT5800.034.5236730
ACGATCG5800.034.5236727
TATCTCG5800.034.5236736
CGATCGT5800.034.5236728
ATCTCGT5850.034.22859637
TATGCCG5850.034.22859643
CGTATGC5900.033.93852241
TCGACTG606.4028427E-933.74785635
Produced by FastQC (version 0.11.2)