FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_48.35100000041936.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_48.35100000041936.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156929
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC12370.7882545609798062No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG5220.3326345035015835No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4340.276558188735033No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT3590.22876587501354115No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2460.1567587890064934No Hit
CTCATGGACTATGCCTTTGAGTACATTGTTAAGAACGGAGGTCTACGCAAG2210.14082801776599607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA1850.11788770717967999No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA1780.11342709123234072No Hit
TCCTTACTCTATGGAAGAAGGAACTTGCGAGATGCAAAAGGATGAATCTGA1690.10769201358576172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2350.044.99968316
CTGCTTG2550.039.70560524
CTTCTGC2650.039.05632821
CCGTCTT2750.038.45427317
GCTTGAA2950.036.6099126
GTCTTCT2800.036.1604619
TATCGCG250.002101653335.99974440
TCTGCTT2850.035.52606223
CGTCTTC2900.034.91354818
TGCTTGA3250.033.92283625
GCCTTGT401.5521016E-533.749768
TTCTGCT3050.033.19648422
TCTTCTG3000.032.99976720
TACCCCG1100.032.72704320
TGAAAAA3250.032.5382329
TTGAAAA3250.032.5382328
TTCTACA352.8094786E-432.142632
CATGGAC708.021743E-1032.142633
TGCCTTG352.8094786E-432.142637
CTTGAAA3350.031.5669427
Produced by FastQC (version 0.11.2)