FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_47.3510000004191c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_47.3510000004191c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149245
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC73124.89932661060672No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG54753.6684646051794028No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT17771.190659653589735No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA6870.4603169285403196Illumina PCR Primer Index 8 (96% over 27bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2880.192971288820396No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT2660.17823042647994908No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2510.16817983852055346No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA2460.16482964253408824No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG2280.1527689369828135No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA2190.14673858420717611Illumina PCR Primer Index 8 (96% over 27bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2160.14472846661529698No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT2150.14405842741800395No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA1960.13132768266943615No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1960.13132768266943615No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1940.1299876042748501No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTC1900.1273074474856779No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1830.12261717310462661No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1720.11524674193440317No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT1670.11189654594793796No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT1650.11055646755335187No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTAT1560.1045261147777145No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA1560.1045261147777145No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGAAAA1550.10385607558042145Illumina PCR Primer Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAC206.9932255E-445.0264933
CCAGGTG253.876326E-544.996319
CCGTCTT14100.044.67719317
CGTCTTC14050.044.67605618
GCTTGAA13800.044.67025826
GCCGTCT14150.044.51931816
TTCTGCT13700.044.5036522
GTCTTCT14150.044.3603219
CTTGAAA14000.044.35350827
TGCTTGA13850.044.3465525
TCTGCTT13900.044.1870323
TTGAAAA14100.044.03894428
CTGCTTG13950.043.86737424
CTTCTGC14250.043.7332621
GAAAAAA14750.043.3184930
TCTTCTG14550.042.83154320
TGAAAAA15050.042.45499429
CAGGTGT301.1357149E-437.49692510
AGCGGTG301.1357149E-437.49692517
AATGCGT301.1357149E-437.49692525
Produced by FastQC (version 0.11.2)