FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_45.351000000418ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_45.351000000418ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213086
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC67303.1583492111166387No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG32541.527082961808847No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT23871.1202049876575655No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA9840.4617853824277522Illumina PCR Primer Index 8 (96% over 27bp)
TACCACAAGGCTTGCATTACGTTCCTGTCTCTTATACACATCTCCGAGCCC2410.11309987516777265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT20250.044.55230316
CCGTCTT20600.043.79534517
TGCTTGA19900.043.52697425
CTTCTGC20900.043.38199621
TGAAAAA20850.043.37812829
CGTCTTC20900.043.2743518
CTGCTTG20250.042.9968624
GCTTGAA20250.042.77465426
TTGAAAA20250.042.6635528
CTTGAAA20500.042.36276227
TTCTGCT20800.042.07625222
GAAAAAA21550.041.8646930
GTCTTCT21750.041.58316819
TCTTCTG21800.041.28138720
TCTGCTT21450.041.11587523
GCCGTCC301.13729155E-437.4972616
TCTAGGG250.002099175536.0142752
CGTCCTC250.0021040235.9973718
CGATGTG458.62894E-735.013883
GCCTGAA1050.034.2832126
Produced by FastQC (version 0.11.2)