FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_43.35100000041887.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_43.35100000041887.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226863
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC43771.929358247047778No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA29381.2950547246576127No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT27381.206895791733337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG23241.024406800580086No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT18330.8079766202509884No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC14210.6263692184269801No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA8300.3658595716357449No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT7460.3288328198075491No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7010.308997059899587No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA5790.2552201108157787No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5670.2499305748403221No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5380.23714752956630214No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC5310.23406196691395245No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4890.21554859099985452No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4630.2040879297196987No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4440.19571283109189244No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4230.1864561431348435No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3990.1758770711839304No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC3610.15912687392831798No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3590.15824528459907522No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3440.15163336462975452No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3360.14810700731278348No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3350.1476662126481621No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3340.14722541798354072No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3270.14413985533119109No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3210.14149508734346278No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTCTTATACACATCT3180.14017270334959867No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3110.137087140697249No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGCT2940.12959363139868554No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG2830.12474489008785036No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT2710.11945535411239383No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2640.11636979146004416No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC2610.11504740746618003No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC2590.11416581813693727No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA2510.11063946081996624No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA2470.10887628216148071No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2430.10711310350299519No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT2390.10534992484450968No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACAC2380.1049091301798883No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACACAT2380.1049091301798883No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2370.10446833551526692No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT2360.10402754085064554No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG2340.1031459515214028No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA2300.10138277286291728No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTGA207.0213055E-445.013
CCGTCTT3650.043.15068417
GCCGTCT3750.041.99999616
GCTTGAA3550.041.83098626
GTCTTCT3800.041.4473719
CGTCTTC3850.040.9090918
TCTGCTT3850.040.32467323
CTGCTTG3850.039.74025724
TTCTGCT3850.039.74025722
TGCTTGA3850.039.1558425
TTGAAAA3900.038.07692328
CTTCTGC4150.037.95180521
ACGGGCC301.1370357E-437.5000043
CTTGAAA4100.036.7682927
TGAAAAA4550.034.61538329
TCTTCTG4700.033.5106420
CTGAAAC4100.032.37804844
GAAAAAA5500.030.68181830
GTCCTTA300.005138654730.0000026
GACAATC300.005138654730.0000027
Produced by FastQC (version 0.11.2)