FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_42.3510000004187a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_42.3510000004187a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316510
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC42371.3386622855517993No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT22980.7260434109506808No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG14510.4584373321538024No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11440.36144197655682286No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA6900.21800259075542638No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5430.17155856055100946No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT4430.13996398218065778No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4270.13490884964140154No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3810.12037534359103977No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3730.11784777732141165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT5450.043.3454316
CCGTCTT5950.039.3248317
CTGCTTG6050.037.55921624
TGAAAAA6350.037.2019829
GTCTTCT6350.036.84767519
TGCTTGA6250.036.35732325
GCTTGAA6200.036.28764726
TTCTGCT6300.036.0687722
CGTCTTC6500.035.9973518
CTACCGT458.678471E-734.997428
CTTCTGC6750.034.99741721
CCCCTCC401.557796E-533.7475129
ATCCCCC401.557796E-533.7475126
CTTGAAA6800.033.41665327
TCTGCTT6850.033.17273723
GCTACCG501.976763E-631.4976797
TCTTCTG7500.031.197720
GAAAAAA7800.030.5746730
TTGAAAA7600.029.8991128
CCCTCCA406.158851E-428.12292530
Produced by FastQC (version 0.11.2)