FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_41.35100000041844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_41.35100000041844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214143
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC52892.469844916714532No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA22951.0717137613650691No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG16870.7877913356962405No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT16840.7863904026748482No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT12830.5991323554820844No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA8120.37918587112350155No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA7330.34229463489350576Illumina PCR Primer Index 8 (96% over 27bp)
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA5210.24329536804845361No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT4230.19753155601630687No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC4200.1961306229949146No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3960.18492315882377663No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA3430.1601733421125136No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3420.1597063644387162No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA3400.15877240909112134No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT3260.15223472165795754No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3060.14289516818200923No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC2960.13822539144403506No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA2930.13682445842264282No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTCTTATACACATCT2910.13589050307504796No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCTGTCTCTTATACAC2870.1340225923798583No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCTTATACACATCTC2740.1279518826204919No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2700.12608397192530224No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC2510.11721139612315135No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2360.11020673101619011No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACT2350.10973975334239269No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCT2270.10600393195201337No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2250.10506997660441854No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2220.1036690435830263No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2160.10086717754024181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC13700.044.99743318
GCCGTCT13750.044.9974316
CCGTCTT13800.044.83439617
GTCTTCT14000.043.7117919
CTGCTTG13600.043.67397724
GCTTGAA13750.043.52478826
TGCTTGA13700.043.51941725
CTTCTGC14000.043.3903821
TCTGCTT13900.042.89323423
TTCTGCT13900.042.73137322
CTTGAAA14200.042.3039227
TTGAAAA14350.041.8617228
TCTTCTG14600.041.76131420
GAAAAAA15900.039.05437530
TGAAAAA15900.038.77137429
GAGTAAT250.002099058136.0147674
CCCACTG250.002103878735.99794435
ACTCGTA250.002103878735.9979448
CCCCACT250.002103878735.99794434
TGCCGTC19150.032.4263515
Produced by FastQC (version 0.11.2)