FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_39.35100000041800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_39.35100000041800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125384
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5782746.1199196069674No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1799114.348720729917694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG90817.242550883685318No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1470.1172398392139348No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1260.10049129075480126No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCTT207.0092315E-445.00000425
GACCCCT207.0092315E-445.00000426
AACCGGG351.2003875E-745.033
ACTGAAA351.2003875E-745.043
GAACCGG351.2003875E-745.032
CCGGGGG351.2003875E-745.035
TGCCGTC57950.044.6505645
ACCGGGG403.4254663E-739.37500434
CGGGGGA403.4254663E-739.37500436
GGCGAAC403.4254663E-739.37500429
AGGCGAA403.4254663E-739.37500428
GAACTGA403.4254663E-739.37500441
CGAACCG403.4254663E-739.37500431
GGGGAAC403.4254663E-739.37500438
AATGAGG403.4254663E-739.37500424
ATGAGGC403.4254663E-739.37500425
ACTTGTC250.002099969736.00000433
GGGGATA250.002099969736.0000048
GAGACCC250.002099969736.00000424
GCGAACC458.61799E-735.030