FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_38.351000000417f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_38.351000000417f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248722
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC6734027.07440435506308No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT3513314.125409091274596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG111434.480102282870031No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8300.3337059045842346No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6420.2581195069193718No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA4680.18816188354870095No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3200.1286576981529579No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3000.12061659201839806No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2690.10815287750983026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGT207.022616E-445.0000046
TGCCGTC69350.044.70800445
CTGTGTG301.1373513E-437.5000049
AGAATAC250.002103941736.05
GCCGTCT352.8140677E-432.14285745
GATTTAA300.00513959630.00000215
TAGCCTG602.4061046E-730.00000234
TCTACTA300.00513959630.00000231
TCTGGTA300.00513959630.0000023
CTGGTTC1050.030.04
TTAGTGT453.4799697E-530.04
ATACCCG453.4799697E-530.019
ATGCCGT104400.029.69827744
TGGTTCA1100.028.6363645
GGTTCAC1100.028.6363646
ACTGTGT406.152455E-428.1250028
CTGAAAC1650.027.27272644
GTACTGG1250.026.9999981
TGAAACA1600.026.71875245
TATGCCG117450.026.41762443
Produced by FastQC (version 0.11.2)