FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_37.351000000417d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_37.351000000417d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC473.1270791749833666No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA211.3972055888223553No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC201.330671989354624No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC171.1310711909514306No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC171.1310711909514306No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG140.9314703925482368No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT130.8649367930805056No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC120.7984031936127743No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA100.665335994677312No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA90.5988023952095809No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA90.5988023952095809No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC70.4657351962741184No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC70.4657351962741184No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT70.4657351962741184No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT70.4657351962741184No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC70.4657351962741184No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC60.39920159680638717No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG60.39920159680638717No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC60.39920159680638717No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT50.332667997338656No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAC50.332667997338656No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA50.332667997338656No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT50.332667997338656No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG50.332667997338656No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG50.332667997338656No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA40.2661343978709248No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCTC40.2661343978709248No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT40.2661343978709248No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG40.2661343978709248No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT40.2661343978709248No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC40.2661343978709248No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC40.2661343978709248No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG40.2661343978709248No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA40.2661343978709248No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC40.2661343978709248No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT40.2661343978709248No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC40.2661343978709248No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC40.2661343978709248No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGG30.19960079840319359No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG30.19960079840319359No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC30.19960079840319359No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC30.19960079840319359No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA30.19960079840319359No Hit
GCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC30.19960079840319359No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA30.19960079840319359No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG30.19960079840319359No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG30.19960079840319359No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT30.19960079840319359No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC30.19960079840319359No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT30.19960079840319359No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT30.19960079840319359No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAG30.19960079840319359No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG30.19960079840319359No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA30.19960079840319359No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC30.19960079840319359No Hit
GGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAA30.19960079840319359No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT30.19960079840319359No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC30.19960079840319359No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA30.19960079840319359No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAC30.19960079840319359No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA30.19960079840319359No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA30.19960079840319359No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC30.19960079840319359No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA30.19960079840319359No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA30.19960079840319359No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG30.19960079840319359No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA20.1330671989354624No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC20.1330671989354624No Hit
AGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTA20.1330671989354624No Hit
CCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGC20.1330671989354624No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC20.1330671989354624No Hit
GGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG20.1330671989354624No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC20.1330671989354624No Hit
CTTATACACATCTCCGAGCCCACGAACTCTGGACCATCTCGTATGCCGTCT20.1330671989354624No Hit
GATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGC20.1330671989354624No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC20.1330671989354624No Hit
GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCT20.1330671989354624No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG20.1330671989354624No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGAC20.1330671989354624No Hit
CCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC20.1330671989354624No Hit
GTACGGGCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCC20.1330671989354624No Hit
CTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTC20.1330671989354624No Hit
GGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC20.1330671989354624No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACG20.1330671989354624No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCAC20.1330671989354624No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATCTGTC20.1330671989354624No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT20.1330671989354624No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCA20.1330671989354624No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTACTTATCTCGTATGCCGTC20.1330671989354624No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTG20.1330671989354624No Hit
TGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCG20.1330671989354624No Hit
ATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCC20.1330671989354624No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACT20.1330671989354624No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAA20.1330671989354624No Hit
CACCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC20.1330671989354624No Hit
CTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTG20.1330671989354624No Hit
GTATCAACGCAGAGTACGGTAAGCAGTGGTATCAACGCAGAGTACGGTAAG20.1330671989354624No Hit
GCTTTGGGCTGCATTCCCAAACAACCCGACTCCGGGAAGACGCCGAGAGCG20.1330671989354624No Hit
AACGGGGAGGAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTC20.1330671989354624No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT20.1330671989354624No Hit
TTTTTGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC20.1330671989354624No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT20.1330671989354624No Hit
AGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCTTA20.1330671989354624No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTACTTATCTCGTATGCCGTC20.1330671989354624No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTCTTATA20.1330671989354624No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA20.1330671989354624No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC20.1330671989354624No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTG20.1330671989354624No Hit
CGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT20.1330671989354624No Hit
CAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTT20.1330671989354624No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG20.1330671989354624No Hit
CCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCT20.1330671989354624No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC20.1330671989354624No Hit
GGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGG20.1330671989354624No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20.1330671989354624No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC20.1330671989354624No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC20.1330671989354624No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG20.1330671989354624No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA20.1330671989354624No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCT20.1330671989354624No Hit
ACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTG20.1330671989354624No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAA20.1330671989354624No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT20.1330671989354624No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA20.1330671989354624No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA20.1330671989354624No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTG20.1330671989354624No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTC20.1330671989354624No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC20.1330671989354624No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGAT20.1330671989354624No Hit
GGAGCGTTAAAAGCAAAGTCAAGATGAACCACCACCTCGAGCTCCACCACG20.1330671989354624No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCA20.1330671989354624No Hit
CACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCC20.1330671989354624No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAG20.1330671989354624No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)