FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_34.3510000004177b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_34.3510000004177b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94662
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC22592.386385244343031No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5260.5556611945659293No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC3630.3834696076567155No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA3410.3602290253744903No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3370.3560034649595403No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA3160.3338192727810526No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT2880.30424034987640236No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2790.2947328389427648No Hit
GTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGAC2330.24613889417083942No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCCC2180.2302930426147768No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA2120.22395470199235173No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAG1970.20810885043628913No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG1900.20071411971012654No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC1870.197544949398914No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC1820.1922629988802265No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTC1750.18486826815406393No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA1670.17641714732416386No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1550.16374046607931378No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC1540.16268407597557627No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG1520.16057129576810125No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTC1440.15212017493820118No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGG1420.15000739473072616No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA1330.14049988379708858No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG1270.13416154317466353No Hit
GATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG1260.13310515307092605No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1250.13204876296718854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG1240.130992372863451No Hit
TCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCT1190.12571042234476348No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1160.12254125203355094No Hit
GTATCAACGCAGAGTACGGGATCCTCCAAGGCTAAATACTCCTGACTGACC1160.12254125203355094No Hit
TATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGACC1150.12148486192981343No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT1150.12148486192981343No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG1130.11937208172233843No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC1110.11725930151486341No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAA1110.11725930151486341No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC1060.11197735099617587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.11092096089243837No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTCTTATAC1040.10986457078870085No Hit
TTTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGT1030.10880818068496334No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT1030.10880818068496334No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTG1010.10669540047748834No Hit
TTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCC1000.10563901037375081No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1000.10563901037375081No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC970.1024698400625383No Hit
GAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAAC960.10141344995880078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTTC302.144554E-644.99947437
ATCTTCG207.000895E-444.9994738
GTATCTT253.863914E-544.9994736
TGCCGTC2600.043.2687245
CTGGTAT356.1882492E-638.57097633
AACTGCG301.1321041E-437.4995616
ACCAACC301.1321041E-437.499562
ACCCGAT301.1321041E-437.499566
GCAAACT301.1321041E-437.4995613
ATGCCGT3000.037.4995644
TATCTCG3050.036.88481536
TCTACTT2950.036.6097430
CTCTACT2950.036.6097429
CCACGAG3100.036.28989820
TATGCCG3100.036.28989843
CACGAGA3100.036.28989821
CCCACGA3100.036.28989819
CTACTTA3050.036.14711831
CCTGTCC250.002097495635.99957741
CGGGGAC250.002097495635.99957717
Produced by FastQC (version 0.11.2)