FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_33.35100000041745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_33.35100000041745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323395
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC89332.762256683003757No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT42421.3117085916603535No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT20650.6385380107917562No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC8820.272731489355123No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8720.26963929559826216No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT8680.26840241809551785No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8300.2566520818194468No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG8220.25417832681395813No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT8160.2523230105598417No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT7750.23964501615671238No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT6560.2028479104500688No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5960.18429474790890396No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA5960.18429474790890396No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC5900.1824394316547875No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5650.17470894726263547No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT5140.15893875910264538No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5130.15862953972695928No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3810.11781258213639666No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3800.11750336276071058No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACA3430.1060622458603256No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3300.10204239397640655No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3290.10173317460072048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9500.044.2868945
GATCCTA250.00210547835.997943
ATGCCGT13350.031.51501344
CGGGGAA300.005131662430.012174
AGGTACC300.00514332129.998257
ACCTCAC300.00514332129.9982511
GGCTAAT300.00514332129.9982520
CAGACGG300.00514332129.998259
TCGACTG852.0190782E-1029.11594835
TGTCGAC852.0190782E-1029.11594833
TATGCCG14450.029.11594643
CGTATGC14500.029.01554741
TCGTATG14550.028.91583440
CTCGTAT14550.028.76120439
TATCTCG14500.028.7052236
GTTCACC554.1346975E-628.6524141
ATCTCGT14600.028.50860837
CCACGAG14800.028.42739320
CGAGACC14650.028.4113123
TAACCTA13250.028.3699935
Produced by FastQC (version 0.11.2)