FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_30.351000000416e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_30.351000000416e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416198
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC205344.933709436374033RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT81491.9579623160130515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG33620.8077886006179751No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8890.2136002575697144No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5400.1297459382313226No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4750.11412837159236709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22000.044.28196345
ATGCCGT29800.032.69138344
TATGCCG32350.030.11447343
GTATGCC32600.029.95254942
CGTATGC32800.029.70131741
TCGTATG32950.029.56610540
CTCGTAT33150.029.38772839
GACCTAT33100.029.1602326
AGACCTA33250.029.02867925
CCTATTC32950.029.0198528
ACCTATT33150.028.98050927
AATCTCG33550.028.97029136
ATCTCGT33750.028.93194237
TCTCGTA33800.028.88914338
ACGAGAC33850.028.84647222
CGAGACC33750.028.79861623
CCACGAG33850.028.78000520
CTATTCA33350.028.67178529
CACGAGA34200.028.61704621
CCCACGA34050.028.6109619