Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pa_30.351000000416e6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 416198 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC | 20534 | 4.933709436374033 | RNA PCR Primer, Index 13 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT | 8149 | 1.9579623160130515 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG | 3362 | 0.8077886006179751 | No Hit |
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA | 889 | 0.2136002575697144 | No Hit |
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT | 540 | 0.1297459382313226 | No Hit |
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC | 475 | 0.11412837159236709 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 2200 | 0.0 | 44.281963 | 45 |
ATGCCGT | 2980 | 0.0 | 32.691383 | 44 |
TATGCCG | 3235 | 0.0 | 30.114473 | 43 |
GTATGCC | 3260 | 0.0 | 29.952549 | 42 |
CGTATGC | 3280 | 0.0 | 29.701317 | 41 |
TCGTATG | 3295 | 0.0 | 29.566105 | 40 |
CTCGTAT | 3315 | 0.0 | 29.387728 | 39 |
GACCTAT | 3310 | 0.0 | 29.16023 | 26 |
AGACCTA | 3325 | 0.0 | 29.028679 | 25 |
CCTATTC | 3295 | 0.0 | 29.01985 | 28 |
ACCTATT | 3315 | 0.0 | 28.980509 | 27 |
AATCTCG | 3355 | 0.0 | 28.970291 | 36 |
ATCTCGT | 3375 | 0.0 | 28.931942 | 37 |
TCTCGTA | 3380 | 0.0 | 28.889143 | 38 |
ACGAGAC | 3385 | 0.0 | 28.846472 | 22 |
CGAGACC | 3375 | 0.0 | 28.798616 | 23 |
CCACGAG | 3385 | 0.0 | 28.780005 | 20 |
CTATTCA | 3335 | 0.0 | 28.671785 | 29 |
CACGAGA | 3420 | 0.0 | 28.617046 | 21 |
CCCACGA | 3405 | 0.0 | 28.61096 | 19 |