FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_29.351000000416d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_29.351000000416d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297808
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC3481411.690082200612476RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT105603.5459087734379198No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG59461.9965884059528287No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA24600.8260355665395155No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT16230.5449820018266803No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC15400.5171116961263633No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10720.3599634663944555No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA10710.35962767957878905No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA10640.3572771718691237No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT9800.3290710793531403No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA9570.3213479825928115No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA7820.2625852898511793No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT7130.23941599957019288No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT7090.238072852307527No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT6440.21624670928920647No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT6150.20650889163487887No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC5700.19139848492988773No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA5690.19106269811422125No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG5370.18031752001289422No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT4540.15244721431257724No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCTGTCTCTTATACAC4280.14371675710524903No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT4270.14338097028958255No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT4200.14103046257991728No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC3830.1286063504002579No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACC3810.12793477676892495No Hit
GGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATAC3720.12491269542792671No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC3520.11819695911459732No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACAC3420.11483909095793264No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC3240.10879492827593616No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCCT3130.10510127330360501No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT3100.1040939128566056No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCCTGTCTCTTATAC3070.10308655240960618No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3070.10308655240960618No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTG3040.10207919196260679No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA2980.10006447106860797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT253.885279E-544.99714317
TGCCGTC36850.044.5087145
GGTGTGG250.002105263835.99771519
TCGTAGA250.002105263835.99771525
ATGCCGT48500.033.81744444
TATGACT352.816442E-432.1408233
GCCGTCT352.816442E-432.1408245
TATCGCC501.9760864E-631.49834
TATGCCG54150.030.2889443
CGTATGC54550.030.14932441
TCGTATG54650.030.09415640
GTATGCC54550.030.06683542
TCTAGGA300.005130145230.0132143
TACAGGG1350.030.0132122
GTATTCG300.00514280229.99809816
CAGCCCC1350.029.99809812
AGCCCCG1350.029.99809813
ATAACCC901.2732926E-1129.99809844
CGTCCCG300.00514280229.99809814
GCGTCCC300.00514280229.99809813
Produced by FastQC (version 0.11.2)